La diversité microbienne d'un sol est difficile à caractériser. Ceci s'explique par une accessibilité plus ou moins importante des populations au sein d'une matrice hétérogène et structurée, mais aussi par l'incapacité à résoudre une information constituée de 100 000 à 1 000 000 d'espèces différentes par gramme de sol. Toutefois, récemment,...
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March 24, 2011 (v1)Conference paperUploaded on: March 25, 2023
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2011 (v1)Journal article
Fungi constitute an important group in soil biological diversity and functioning. However, characterization and knowledge of fungal communities is hampered because few primer sets are available to quantify fungal abundance by real-time quantitative PCR (real-time Q-PCR). The aim in this study was to quantify fungal abundance in soils by...
Uploaded on: December 4, 2022 -
June 6, 2012 (v1)Conference paper
La diversité microbienne d'un sol (que l'on estime à 100000 à 1000000 d'espè ces différentes par gramme de sol) est difficile à caractériser. Toutefois, d'importantes avancé es en biologie moléculaire (comme le développement du pyrosé quençage), ont permis d'obtenir plusieurs centaines de milliers de sé quences à partir d'un ADN...
Uploaded on: December 4, 2022 -
March 2007 (v1)Journal article
Plant residues, mainly made up of cellulose, are the largest fraction of organic carbon material in terrestrial ecosystems. Soil microorganisms are mainly responsible for the transfer of this carbon to the atmosphere, but their contribution is not accurately known. The aim of the present study was to identify bacterial populations that are...
Uploaded on: December 4, 2022 -
September 2011 (v1)Journal article
Fungi constitute an important group in soil biological diversity and functioning. However, characterization and knowledge of fungal communities is hampered because few primer sets are available to quantify fungal abundance by real-time quantitative PCR (real-time Q-PCR). The aim in this study was to quantify fungal abundance in soils by...
Uploaded on: February 28, 2023 -
April 23, 2012 (v1)Publication
La diversité microbienne d'un sol (que l'on estime à 100000 à 1000000 d'espè ces différentes par gramme de sol) est difficile à caractériser. Toutefois, d'importantes avancé es en biologie moléculaire (comme le développement du pyrosé quençage), ont permis d'obtenir plusieurs centaines de milliers de sé quences à partir d'un ADN...
Uploaded on: March 25, 2023 -
November 14, 2011 (v1)Publication
La diversité microbienne d'un sol (que l'on estime entre 100 000 et 1 000 000 d'espèces différentes par gramme de sol) est difficile à caractériser. Toutefois, d'importantes avancées en biologie moléculaire, comme le développement du pyroséquençage, ont permis d'obtenir plusieurs centaines de milliers de séquences à partir d'un ADN...
Uploaded on: February 22, 2023 -
November 28, 2011 (v1)Publication
La diversité microbienne d'un sol (que l'on estime entre 100 000 et 1 000 000 d'espèces différentes par gramme de sol) est difficile à caractériser. Toutefois, d'importantes avancées en biologie moléculaire, comme le développement du pyroséquençage, ont permis d'obtenir plusieurs centaines de milliers de séquences à partir d'un ADN...
Uploaded on: December 4, 2022 -
January 31, 2012 (v1)Conference paper
National audience
Uploaded on: December 4, 2022 -
April 23, 2012 (v1)Conference paper
La diversité microbienne d'un sol (que l'on estime entre 105 et 106 espèces différentes par gramme de sol) est difficile à caractériser. Cependant, les techniques de séquençage haut-débit comme le pyroséquençage permettent maintenant d'étudier la diversité des communautés microbiennes du sol en se basant sur un marqueur...
Uploaded on: December 4, 2022 -
June 26, 2011 (v1)Conference paper
National audience
Uploaded on: December 4, 2022 -
August 22, 2012 (v1)Conference paper
During the last two decades, novel molecular and robust methods were developed that were well-suited to characterize soil microbial communities, as they provided access to previously hidden genetic resources. These methods were based essentially on soil DNA characterization and most efforts were devoted to optimize the ...
Uploaded on: February 22, 2023 -
August 22, 2012 (v1)Conference paper
During the last two decades, novel molecular and robust methods were developed that were well-suited to characterize soil microbial communities, as they provided access to previously hidden genetic resources. These methods were based essentially on soil DNA characterization and most efforts were devoted to optimize the ...
Uploaded on: December 4, 2022 -
August 2013 (v1)Journal article
The turnover of organic matter in soil depends on the activity of microbial decomposers. However, little is known about how modifications of the diversity of soil microbial communities induced by fresh organic matter (FOM) inputs can regulate carbon cycling. Here, we investigated the decomposition of two 13C labeled crop residues (wheat and...
Uploaded on: December 4, 2022 -
August 19, 2012 (v1)Publication
Soil is one of the most important reservoirs of microbiological diversity on our planet and, above all, one of the last bastions of such biodiversity. Since two decades, numerous molecular tools have been developed to assess accurately this huge diversity directly from soil DNA. In this context, 16S rRNA gene is widely used to study microbial...
Uploaded on: December 4, 2022 -
January 2012 (v1)Journal article
Three soil DNA extraction procedures (homemade protocols and commercial kit) varying in their practicability were applied to contrasting soils to evaluate their efficiency in recovering: (i) soil DNA and (ii) bacterial diversity estimated by 16S rDNA pyrosequencing. Significant differences in DNA yield were systematically observed between...
Uploaded on: February 28, 2023