PERES , Chiara

The most recent advances in optical microscopy are mainly focused towards superresolution, using fluorescence stochastic/targeted read-out methods [1]. Since the demand is growing for imaging thick biological specimens as cell aggregates (i.e., tumor spheroids), tissues/organs (i.e. ligaments, meniscus) and small organisms (i.e. zebrafish),...

We propose and demonstrate a novel subtraction microscopy algorithm, exploiting fluorescence emission difference or switching laser mode and their derivatives for image enhancement. The key novelty of the proposed approach lies in the weighted subtraction coefficient, adjusted pixel-by-pixel with respect to the intensity distributions of...

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Sensitivity of the amplitude and phase measurements in interferometric microscopy is influenced by factors such as instrument design and environmental interferences. Through development of a theoretical framework followed by experimental validation, we show photon shot noise is often the limiting factor in interferometric microscopy...

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