PESCE , Luca

Expansion microscopy (ExM) is a super-resolution imaging method that does not require any special optical microscope(1). The key point resides on the possibility of uniformly chemically expanding a sample, thus increasing the relative distances among objects of interest as fluorescent molecules labeling specific components. ExM is highly...

Expansion microscopy is a super-resolution method that allows expanding uniformly biological samples, by increasing the relative distances among fluorescent molecules labeling specific components. One of the main concerns in this approach regards the isotropic behavior at the nanoscale. The present study aims to determine the robustness of such...

Hutchinson-Gilford Progeria Syndrome (HGPS) is a lethal congenital disorder characterized by premature aging symptom in children and early death. It is often used as model to understand the molecular mechanism of aging. This condition is caused by a single nucleotide substitution which leads a cryptic splice site in the C-terminal Lamin A....

Three-dimensional imaging of cells or tissues is a crucial method in cellular and molecular biology due to its capability to identify and localise diverse biomolecules at the nanoscale. Recently, an approach has become very popular which can physically and isotropically expand a biological specimen through a chemical process by embedding the...

The possibility of integrating different light-matter interactions to form images in optical microscopy is the starting point for the design and realization of LIQUITOPY®, a ''liquid tunable microscope''. This could represent a new paradigm in data collection and image formation with a potential high impact in biophysics. Liquid takes...

Circular Dichroism (CD) resolved scanning microscopy has proven to be an interesting label free technique to study chiral biological systems. This technique is based on the measurement of the difference of intensity between circular left and right polarization states after interaction with the sample. It was demonstrated in early works that far...

Photodynamic therapy (PDT) is a tumor treatment that uses a combination of otherwise non-toxic chemicals, termed photosensitizers (PSs) and visible light to produce reactive oxygen species capable of destroying tumor cells [Dougherty TJ (1993)][Agostinis P. et al.(2011)].PS molecules are often insoluble in water and form aggregates, which...

The possibility of integrating different light-matter interaction ways for producing images in optical microscopy is the starting point for the design and realization of a brand new optical microscope that has been named "liquid tunable microscope". This represents a new paradigm in data collection and image formation with a potential high...

The fact that the size of many molecular complexes is in the range of 10 to 100nm has generated a growing interest for the development of super-resolved fluorescence microscopy techniques. Optical super-resolution is generally achieved by switching on-off the fluorophores by stochastic or targeted approaches [1]. For instance, in stimulated...