SCANAROTTI , Chiara

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meeting report on From Cells to QSAR: Alternative predictive models in toxicology, hosted by The Italian Association for In Vitro Toxicology CELLTOX in collaboration with the Laboratory of Analysis and research in Physiopathology (LARF), Department of Experimental Medicine (DIMES) of the University of Genova, in April 2016. The meeeting was a a...

. In vitro animal models of the nervous system have been in use for many years provide an important tool for functional studies at cellular and molecular levels. Aim of our study was to generate standardized neural precursor populations from human Adipose-derived Stem Cells (hADSC), by tissue, routinely discarded after liposuction surgeries....

To underline the importance of both the ethical issues of alternative approaches to animal testing and the life sciences in the 21st century, LARF-DIMES, at the University of Genova (Italy), is engaged since 2008 in organization of training courses for dissemination of international resources using the over twenty years of experience of its...

Our complementary methods can represent an high content sensitive tool to assess the safety of chemical mixtures, that can act on skin, as well on eye and neural functions. Aim of this study was to assess the toxic potential of two mixtures (Cat. EWC160506, one inorganic and one organic), by different in vitro approaches.

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Environmental pollutants exhibit a variety of adverse biological effects on human health. Polychlorinated Biphenyls (PCBs) can modulate differentiation, metabolism and function of the white adipose tissue (WAT) in humans through a wide range of mechanisms able to exert their effects in the whole organism. Moreover, interactions among different...

The presence of waste in the environment has frequently been indicated as a significant risk to human health. Therefore, landfill sites and the disposal of urban solid and non-hazardous waste by incineration are subject to much environmental monitoring, in addition to the regulations already in place. However, little action has been taken, and...

Oxidation antitumor therapy is developed by delivering excess oxidative stress into tumor cells or targetedly disrupting the antioxidant systems. In present study, several markers related to oxidative stress, proliferation and angiogenesis were evaluated in human DLD-1 colon carcinoma, and HECV normal endothelial cells, during exposure to...

On September 29-30, 2016, the 8th edition of the training course on "Alternative Approach to Animal Testing and Cell Cultures, According to European Laws", was held in Genoa, chaired by Prof. Anna Maria Bassi and organized and hosted by the team of Laboratory Analysis and Research in Physiopathology (LARF), Department of Experimental ...

A reactive oxygen species-mediated targeting system has been used to selectively kill cancer cells. Two different cell lines, normal and cancer cells, have been cultured and treated with a peroxide olive oil (K600) in simple solution and in form of nanoemulsion (N-K600). Preliminary results of both treatments have been compared.

The oxidant/antioxidant status of patients could influence tumor growth and proliferation and subsequently overall survival. Lipid hydroperoxides (LOOHs) accumulate, not in young and proliferating cells, but in aging cells, suggesting that this intracellular increasing should restore the capability of tumour cells to age and die. Potential...

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According to European laws animal testing in cosmetic industry will be prohibited in a few years and it will be replaced by alternative methods based on cell and tissue culture. Many ingredients of cosmetic formulations are potentially causes of skin inflammation and sensibilization. Since cytotoxicity is known, among other factors, to trigger...

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2D- compared 3D- human cell models: cytotoxic effects of humid lightweight coal ash derived from the disposal of waste

Libro rivolto a studenti di discipline scientifiche, pubblicato da Aracne editrice s.r.l., Roma, 2008

A canal sealing cement must show high biocompatibility and fill completely root canal. Sometimes some components of such sealers can act as irritative stimulus for buccal mucosa. Novel alternative testing strategies are requested for evaluating cellular specific adverse effects. Cefinal Endocem® is a dental cement that undergo to polymerization...

The presence of waste in environment represents a significant risk to huma health.. The potential toxic effects of humid lightweight coal ash (EWC100102) derived from waste disposal were investigated in vitro on human keratynocyte and andotelial cell lines, comparing different approac: direct/indirect contact, 2D/3D models.Cyotoxic potential...

Oxidative stress exerts major role in the pathogenesis of side effects of many antineoplastic drugs, including ototoxicity of cisplatin. In particular, increased levels of reactive oxygen species (ROS) represent one of the molecular mechanisms underlying the apoptosis of different types of hearing cells. Antioxidants and ROS scavengers may thus...