We propose a 3D super-resolution approach to improve both lateral and axial spatial resolution in Total Internal Reflectance Fluorescence (TIRF) imaging applications. Our approach, called 3D-COL0RME (3D-Covariance-based ℓ 0 super-Resolution Microscopy with intensity Estimation) improves both lateral and axial resolution by combining...
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March 28, 2022 (v1)Conference paperUploaded on: December 3, 2022
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April 29, 2014 (v1)Conference paper
We propose a new model for the reconstruction of biological struc- tures using Multiple-Angle Total Internal Reflection Fluorescence Microscopy (MA-TIRFM). This recent microscopy technique allows the visualization of sub-cellular structures around the plasma mem- brane which is of fundamental importance in the comprehension of exchanges...
Uploaded on: October 11, 2023 -
November 25, 2023 (v1)Publication
Image super-resolution techniques exploiting the stochastic fluctuations of image intensities have become a powerful tool in fluorescence microscopy. Compared to other approaches, these techniques can be applied under standard acquisition settings and do not require special microscopes nor fluorophores. Most of these approaches can be...
Uploaded on: November 30, 2023 -
October 27, 2014 (v1)Conference paper
Super-resolution microscopy techniques allow to overstep the diffraction limit of conventional optics. Theses techniques are very promising since they give access to the visualisation of finer structures which is of fundamental importance in biology. In this paper we deal with Multiple-Angle Total Internal Reflection Mi- croscopy (MA-TIRFM)...
Uploaded on: March 25, 2023 -
April 29, 2014 (v1)Conference paper
We propose a new model for the reconstruction of biological struc- tures using Multiple-Angle Total Internal Reflection Fluorescence Microscopy (MA-TIRFM). This recent microscopy technique allows the visualization of sub-cellular structures around the plasma mem- brane which is of fundamental importance in the comprehension of exchanges...
Uploaded on: December 3, 2022 -
August 20, 2012 (v1)Journal article
Membrane lipids have been implicated in many critical cellular processes, yet little is known about the role of asymmetric lipid distribution in cell morphogenesis. The phosphoinositide bis-phosphate PI(4,5)P(2) is essential for polarized growth in a range of organisms. Although an asymmetric distribution of this phospholipid has been observed...
Uploaded on: December 4, 2022 -
April 8, 2019 (v1)Conference paper
Total internal reflection fluorescence microscopy (TIRF) produces 2D images of the fluorescent activity integrated over a very thin layer adjacent to the glass coverslip. By varying the illumination angle (multi-angle TIRF), a stack of 2D images is acquired from which it is possible to estimate the axial position of the observed biological...
Uploaded on: December 4, 2022 -
September 28, 2022 (v1)Publication
We propose FluoGAN, an unsupervised hybrid approach combining the physical modelling of fluorescence microscopy timelapse acquisitions with a Generative Adversarial Network (GAN) for the problem of image deconvolution. Differently from standard approaches combining a least-square data fitting term based on one (longtime exposure) image with...
Uploaded on: December 3, 2022 -
March 29, 2023 (v1)Publication
We propose FluoGAN, an unsupervised hybrid approach combining the physical modelling of fluorescence microscopy timelapse acquisitions with a Generative Adversarial Network (GAN) for the problem of image deconvolution. Differently from standard approaches combining a least-square data fitting term based on one (longtime exposure) image with...
Uploaded on: April 14, 2023 -
February 23, 2017 (v1)Journal article
Tissue morphogenesis relies on proper differentiation of morphogenetic domains, adopting specific cell behaviours. Yet, how signalling pathways interact to determine and coordinate these domains remains poorly understood. Dorsal closure (DC) of the Drosophila embryo represents a powerful model to study epithelial cell sheet sealing. In this...
Uploaded on: December 4, 2022 -
April 2011 (v1)Journal article
The transition from immotile epithelial cells to migrating cells occurs in all organisms during normal embryonic development, as well as during tumour metastasis. During Drosophila oogenesis, border cells (BCs) are recruited and delaminate from the follicular epithelium. This process is triggered by the polar cells (PCs), which secrete the...
Uploaded on: December 4, 2022 -
December 15, 2019 (v1)Conference paper
Super-resolution techniques for fluorescence microscopy areinvaluable tools for studying phenomena that take place atsub-cellular scales, thanks to their capability of overcominglight diffraction. Yet, achieving sufficient temporal resolutionfor imaging live-cell processes remains a challenging prob-lem. Exploiting the temporal...
Uploaded on: December 4, 2022 -
August 2018 (v1)Journal article
This work is devoted to the experimental study of the magnetic separation of medium sized nanoparticles (40-70 nm) which are separated from the suspending fluid when the suspension flows through a micropillar array magnetized by an external magnetic field. The nanoparticle accumulation around micropillars is visualized and described by the...
Uploaded on: December 4, 2022 -
July 2017 (v1)Conference paper
The present work is focused on experimental study of magnetic separation of magnetic nanoparticles of a size range of 20-100 nm on ordered arrays of nickel micro-pillars when a dilute suspension of nanoparticles (ferrofluid) flows through a flat narrow channel with micro-pillars spanning the channel width and magnetized by an external magnetic...
Uploaded on: February 28, 2023 -
February 16, 2022 (v1)Journal article
To overcome the physical barriers caused by light diffraction, super-resolution techniques are often applied in fluorescence microscopy. State-of-the-art approaches require specific and often demanding acquisition conditions to achieve adequate levels of both spatial and temporal resolution. Analyzing the stochastic fluctuations of the...
Uploaded on: December 3, 2022 -
July 14, 2015 (v1)Journal article
The phospholipid phosphatidylinositol-4-phosphate [PI(4)P], generated at the Golgi and plasma membrane, has been implicated in many processes, including membrane traffic, yet its role in cell morphology changes, such as the budding to filamentous growth transition, is unknown. We show that Golgi PI(4)P is required for such a transition in the...
Uploaded on: February 28, 2023 -
April 13, 2021 (v1)Conference paper
Super-resolution light microscopy overcomes the physical barriers due to light diffraction, allowing for the observation of otherwise indistinguishable subcellular entities. However, the specific acquisition conditions required by state-of-the-art super-resolution methods to achieve adequate spatio-temporal resolution are often very...
Uploaded on: December 3, 2022 -
2012 (v1)Journal article
Hedgehog (Hh) proteins are secreted molecules that function as organizers in animal development. In addition to being palmitoylated, Hh is the only metazoan protein known to possess a covalently-linked cholesterol moiety. The absence of either modification severely disrupts the organization of numerous tissues during development. It is...
Uploaded on: December 2, 2022 -
April 13, 2016 (v1)Conference paper
This communication presents a pipeline for Multi-Angle TIRF calibration from the measurement of the incident angle to the model validation. This problem is of major importance when dealing with 3D reconstruction methods from a set of MA-TIRF acquisitions since the reconstruction accuracy highly depends on the agreement between the theoretical...
Uploaded on: February 28, 2023 -
2012 (v1)Journal article
Hedgehog (Hh) proteins are secreted molecules that function as organizers in animal development. In addition to being palmitoylated, Hh is the only metazoan protein known to possess a covalently-linked cholesterol moiety. The absence of either modification severely disrupts the organization of numerous tissues during development. It is...
Uploaded on: December 4, 2022 -
December 2019 (v1)Journal article
High resolution imaging of molecules at the cell-substrate interface is required for understanding key biological processes. Here we propose a complete pipeline for multi-angle total internal reflection fluorescence microscopy (MA-TIRF) going from instrument design and calibration procedures to numerical reconstruction. Our custom setup is...
Uploaded on: December 4, 2022 -
November 15, 2010 (v1)Journal article
Cellular fibronectin (cFN) variants harboring extra FN type 3 repeats, namely extra domains B and A, are major constituents of the extracellular matrix around newly forming blood vessels during development and angiogenesis. Their expression is induced by angiogenic stimuli and their assembly into fibrillar arrays is driven by cell-generated...
Uploaded on: December 3, 2022 -
December 2020 (v1)Journal article
International audience
Uploaded on: December 4, 2022 -
December 2022 (v1)Journal article
All epithelia are surrounded by a specialized extracellular matrix, the basement membrane (BM). During development, this BM contributes to the morphogenesis of epithelial organs through different functions, the most recently added one being the shaping of the tissue. Seminal studies highlight the importance of the mechanical properties of the...
Uploaded on: February 22, 2023 -
April 4, 2018 (v1)Conference paper
The role of the extracellular matrix (ECM) in the evolution of certain diseases (e.g. fibrosis, cancer) is generally accepted but yet to be completely understood. A numerical model that captures the physical properties of the ECM, could convey certain connections between the topology of its constituents and their associated biological features....
Uploaded on: December 4, 2022