. In vitro animal models of the nervous system have been in use for many years provide an important tool for functional studies at cellular and molecular levels. Aim of our study was to generate standardized neural precursor populations from human Adipose-derived Stem Cells (hADSC), by tissue, routinely discarded after liposuction surgeries....
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2017 (v1)PublicationUploaded on: April 14, 2023
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2016 (v1)Publication
To underline the importance of both the ethical issues of alternative approaches to animal testing and the life sciences in the 21st century, LARF-DIMES, at the University of Genova (Italy), is engaged since 2008 in organization of training courses for dissemination of international resources using the over twenty years of experience of its...
Uploaded on: April 14, 2023 -
2014 (v1)Publication
Our complementary methods can represent an high content sensitive tool to assess the safety of chemical mixtures, that can act on skin, as well on eye and neural functions. Aim of this study was to assess the toxic potential of two mixtures (Cat. EWC160506, one inorganic and one organic), by different in vitro approaches.
Uploaded on: April 14, 2023 -
2013 (v1)Publication
The presence of waste in the environment has frequently been indicated as a significant risk to human health. Therefore, landfill sites and the disposal of urban solid and non-hazardous waste by incineration are subject to much environmental monitoring, in addition to the regulations already in place. However, little action has been taken, and...
Uploaded on: March 27, 2023 -
2017 (v1)Publication
On September 29-30, 2016, the 8th edition of the training course on "Alternative Approach to Animal Testing and Cell Cultures, According to European Laws", was held in Genoa, chaired by Prof. Anna Maria Bassi and organized and hosted by the team of Laboratory Analysis and Research in Physiopathology (LARF), Department of Experimental ...
Uploaded on: March 27, 2023 -
2015 (v1)Publication
A reactive oxygen species-mediated targeting system has been used to selectively kill cancer cells. Two different cell lines, normal and cancer cells, have been cultured and treated with a peroxide olive oil (K600) in simple solution and in form of nanoemulsion (N-K600). Preliminary results of both treatments have been compared.
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2014 (v1)Publication
The presence of waste in environment represents a significant risk to huma health.. The potential toxic effects of humid lightweight coal ash (EWC100102) derived from waste disposal were investigated in vitro on human keratynocyte and andotelial cell lines, comparing different approac: direct/indirect contact, 2D/3D models.Cyotoxic potential...
Uploaded on: April 14, 2023 -
2016 (v1)Publication
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Uploaded on: April 14, 2023 -
2019 (v1)Publication
A physiologically relevant in vitro human-based model could be the 'gold standard' to clarify the pathological steps involved in glaucoma onset. In this regard, human 3D cultures may represent an excellent starting point to achieve this goal. Indeed, the 3D matrix allows to re-create the in vivo-like tissue architecture, maintaining its...
Uploaded on: April 14, 2023 -
2016 (v1)Publication
The oxidation therapy, an unique antitumor strategy, is based on delivering excess oxidative stress or disrupting the antioxidative defense systems into tumor cells. Lipid-hydroperoxides (LOOHs) derived from polyunsaturated fatty acids, are stable intermediates of oxidative process, and at non-toxic concentrations can act as signalling...
Uploaded on: April 14, 2023