VICIDOMINI , G

We discuss the properties of signal strength and integrated intensity in two-photon excitation confocal microscopy and image scanning microscopy. The resolution, optical sectioning and background rejection are all improved over nonconfocal two-photon microscopy. Replacing the pinhole of confocal two-photon microscopy with a detector array...

Fluorescence lifetime in biological samples is a useful feature to demultiplex the fluorescence signal of spectrally overlapping fluorophores. A problem that frequently occurs when multiple fluorophores are in use is the possible spectral overlap. Therefore, selected fluorophores should be chosen to avoid this issue, consequently constraining...