DE GRAEVE , Fabienne

Neuronal cells rely on macro- and micro-cellular compartmentalization to rapidly process information, and respond locally to external stimuli. Such a cellular organization is achieved via the assembly of neuronal rib- onucleoprotein (RNP) granules, dynamic membrane-less organelles enriched in RNAs and associated regulatory proteins. In this...

We introduce SPADE (small particle detection), a novel method to detect and characterize objects only a few pixel large on microscopy images, based on the use of a dictionary of shapes (Figure 1). It involves the minimization of an energy function embedding a prior and a data fidelity term to match shapes of the dictionary with patterns in...

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In vivo, RNAs and proteins are frequently packaged into diverse dynamic macromolecular structures named mRNP granules. These assemblies form upon phase separation of individual RNA and protein components, a process involving the establishment of multivalent weak interactions and their regulations via post-translational modifications. Defects in...

Stress granules (SGs) are cytoplasmic ribonucleoprotein condensates that dynamically and reversibly assemble in response to acute or chronic stress. They are thought to contribute to the adaptive stress response by storing translationally inactive mRNAs as well as signaling molecules. Recent work has shown that SG composition and properties...

Stress Granules (SGs) are macromolecular assemblies induced by stress and composed of proteins and mRNAs stalled in translation initiation. SGs play an important role in the response to stress and in the modulation of signaling pathways. Furthermore, these structures are related to the pathological ribonucleoprotein (RNP) aggregates found in...

Fragile X syndrome (FXS) is the most frequent inherited cause of intellectual disability and the best-studied monogenic cause of autism. FXS results from the functional absence of the fragile X mental retardation protein (FMRP) leading to abnormal pruning and consequently to synaptic communication defects. Here we show that FMRP is a substrate...

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