ILUNDÁIN LARRAÑETA , María Anunciación Ana

The effect of BaCI2 on galactose transport across isolated rat small intestine has been investigated. The addition of 5 mM BaCI2 or theophylline (3 mM) to the bathing solutions increased .cell water free sugar accumulation and decreased mucosa! to serosa! sugar fluxcs. Howevcr the effects of BaCI2 werc smaller than those induced by...

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Sugar absorption by the small intestine has been studied in rat and hamster in vivo, with luminal perfusion, during 1 minute successive periods. Transport is calculated as the difference between absorption and diffusion. The diffusion component is evaluated in the presence of phlorizin or as absorption of sorbose. The resulting KT values for...

Iodide secretion across different regions of rat small inlestine ha.s been investigated in vitro using the standard Wilson- Wisema technique. Net I secretion was observed along the entire small intestine, being significantly higher in the central region. Anaerobic conditions, ouabain {2 mM) and Na• free Ringer solution prevented net I·...

A simple technique to study intestinal absorption in vivo, a modification of that of SoLS and PONZ (34), is described. It uses a perfusi on pump, with or without recycling of the solution along the intestinal lumen, and allows the carrying out of a ser ies of successive absorption periods with high comparative value on the same...

Na+ , e¡- and water transpon werc studied in jejunum , caecum and colon after either 50 % or 80 % of small bowel resection (SBR). Four weeks after surgery, dry and wet weights, ner absorprion in vivo of sodiu m , chloride and water were detem1ined . There was a significant intestinal growth after 50 % or 80 % SBR exccpt for the colon w hich...

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This study was undertaken to investigate the presence of a Na+{single bond}H+ antiporter in brush-border membrane vesicles (BBMV) isolated from chick small intestine. An outwardly directed proton gradient (pH 5.5 inside, 7.5 outside) stimulated Na+ uptake into BBMV and resulted in a transient accumulation. No accumulation was observed in the...

Kinctics of Glycylsarcosinc (Gly-Sar) uptake by isolated chicken enterocytes was studied by measuring irs intracellular concentration, and by discriminat ing betwccn the saturable and rhe diffusive components of the total uptake. The diffusive component was greater at pH 6.0 than at pH 7.4, and the J max was also increascd by lowering...

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The low renal excretion of betaine indicates that the kidney efficiently reabsorbs the betaine filtered by the glomeruli but the mechanisms involved in such a process have been scarcely investigated. We have detected concentrative and non-concentrative betaine transport activity in brush-border membrane vesicles (BBMV) from rat renal cortex and...

Creatine is involved in brain ATP homeostasis and it may also act as neurotransmitter. Creatine transport was measured in synaptosomes obtained from the diencephalon and telencephalon of suckling and 2 month-old rats. Synaptosomes accumulate [14C]-creatine and this accumulation was Na+- and Cl--dependent and inhibited by high external K+. The...

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The effect of trifluoperazine and RMI 12330A on D-Galactose accumulation was studied in isolated rat intestinal mucosa. Both drugs inhibited the theophylline and phloretin-induced increase in tissue sugar accumulation in a concentration-dependent fashion, with IC50 values close to 10- 6 M. These findings suggest that calmodulin might mediate...

It is well known that surgical resection of the small bowel results in adaptive changes in the residual intestine (7, 13, 14). The entero-hepatic circulation of bile saits, which is responsible for main­ taining constant bile salt pool, is also impaired by intestinal resection. Bile salts are largely reabsorbed by an active...

Este trabajo describe la actividad docente desarrollada por los profesores de la asignatura de Fisiología Celular de la Facultad de Farmacia, durante el Curso Académico 1997-98, coincidiendo con la implantación del Nuevo Plan de Estudios de la Licenciatura de Farmacia. El desarrollo de la aplicación de la actividad tuvo como objetivo aplicar...

D-mannose transport and metabolism has been studied in enterocytes isolated from chicken small intestine. In the presence of Na+, the mannose taken up by the cells either remains free, is phosphorylated, is catabolized to H2O, or becomes part of membrane components. The mannose remaining free in the cytosol is released when the cells are...

Intestinal myofibroblasts secrete substances that control organogenesis and wound repair of the intestine. The myofibroblasts of the rat small intestine express reelin and the present work explores whether reelin regulates crypt-villus unit homeostasis using normal mice and mice with the reelin gene disrupted (reeler). The results reveal that...

The ontogeny of intestinal CRT, AGAT and GAMT was investigated in foetuses, newborn, suckling, weaning and adult rats. In the colon, CRT mediates creatine transport because it was Na+- and Cl—dependent and inhibited by creatine and GPA. In addition, Northern assays showed two CRT transcripts (2.7-kb and 4.2-kb) and the in situ hybridisation...

We recently demonstrated that the mucosa of the small intestine of the rat expresses reelin and some components of its signaling system. The current study evaluates whether reelin affects the intestinal gene expression profile using microarray analysis and reeler mice, a natural mutant in which reelin is not expressed. The effect of the...