LANZANÒ , Luca

Expansion microscopy is a super-resolution method that allows expanding uniformly biological samples, by increasing the relative distances among fluorescent molecules labeling specific components. One of the main concerns in this approach regards the isotropic behavior at the nanoscale. The present study aims to determine the robustness of such...

Hutchinson-Gilford Progeria Syndrome (HGPS) is a lethal congenital disorder characterized by premature aging symptom in children and early death. It is often used as model to understand the molecular mechanism of aging. This condition is caused by a single nucleotide substitution which leads a cryptic splice site in the C-terminal Lamin A....

Tuesday, February 20, 2018 533a accessibility, spatiotemporal confinement and oligomerization states from a single analysis of a few seconds in living cells. The combination of fast sampling speed (down to 1.23 μs per frame) and super-resolution capability opens the doors to the investigation of spatiotemporal dynamics that are not accessible...

Raster image correlation spectroscopy (RICS) is a powerful method for measuring molecular diffusion in live cells directly from images acquired on a laser scanning microscope. However, RICS only provides single average diffusion coefficients from regions with a lateral size on the order of few micrometers, which means that its spatial...

The structural state of DNA in mammalian nucleus of living cells corresponds to varying levels of chromatin organization, ranging from the nucleosomes to higher order structures. This varying degree of hierarchical compaction is integral to genome functions, allowing or preventing access to several genetic nuclear factors (epigenetic,...

The precise localization of nanometric objects in three dimensions is essential to identify functional diffusion mechanisms in complex systems at the cellular or molecular level. However, most optical methods can achieve high temporal resolution and high localization precision only in two dimensions or over a limited axial (z) range. Here we...

Epigenetic dysregulation of chromatin is one of the hallmarks of cancer development and progression, and it is continuously investigated as a potential general bio-marker of this complex disease. One of the nuclear factors involved in gene regulation is the unique DEK protein-a histone chaperon modulating chromatin topology. DEK expression...

Stimulated emission depletion (STED) microscopy is a powerful bio-imaging technique since it provides molecular spatial resolution whilst preserving the most important assets of fluorescence microscopy. When combined with twophoton excitation (2PE) microscopy (2PE-STED), the sub-diffraction imaging ability of STED microscopy can be achieved...

The nucleus is the cellular organelle that harbors the genetic information into DNA, which forms with histone proteins the fundamental unit of chromatin. Chromatin is organized by physical and molecular forces, like phase separation and molecular crowding, into functional domains whose formation and mobility correlate to chromatin compaction...

Confocal fluorescence microscopy is a well-established imaging technique capable of generating thin optical sections of biological specimens. Optical sectioning in confocal microscopy is mainly determined by the size of the pinhole, a small aperture placed in front of a point detector. In principle, imaging with a closed pinhole provides the...

The possibility of integrating different light-matter interactions to form images in optical microscopy is the starting point for the design and realization of LIQUITOPY®, a ''liquid tunable microscope''. This could represent a new paradigm in data collection and image formation with a potential high impact in biophysics. Liquid takes...

The observation of molecular diffusion at different spatial scales, and in particular below the optical diffraction limit (<200 nm), can reveal details of the subcellular topology and its functional organization. Stimulated-emission depletion microscopy (STED) has been previously combined with fluorescence correlation spectroscopy (FCS) to...

The molecular mechanisms that underlie oncogene-induced genomic damage are still poorly understood. To un-derstand how oncogenes affect chromatin architecture, it is important to visualize fundamental processes such as DNA replica-tion and transcription in intact nuclei and quantify the alterations of their spatiotemporal organization induced...

The possibility of integrating different light-matter interaction ways for producing images in optical microscopy is the starting point for the design and realization of a brand new optical microscope that has been named "liquid tunable microscope". This represents a new paradigm in data collection and image formation with a potential high...

In point-scanning microscopy, optical sectioning is achieved using a small aperture placed in front of the detector, i.e. the detection pinhole, which rejects the out-of-focus background. The maximum level of optical sectioning is theoretically obtained for the minimum size of the pinhole aperture, but this is normally prevented by the dramatic...