ONETO , Michele

The structural state of DNA in mammalian nucleus of living cells corresponds to varying levels of chromatin organization, ranging from the nucleosomes to higher order structures. This varying degree of hierarchical compaction is integral to genome functions, allowing or preventing access to several genetic nuclear factors (epigenetic,...

Epigenetic dysregulation of chromatin is one of the hallmarks of cancer development and progression, and it is continuously investigated as a potential general bio-marker of this complex disease. One of the nuclear factors involved in gene regulation is the unique DEK protein-a histone chaperon modulating chromatin topology. DEK expression...

Circular Dichroism (CD) resolved scanning microscopy has proven to be an interesting label free technique to study chiral biological systems. This technique is based on the measurement of the difference of intensity between circular left and right polarization states after interaction with the sample. It was demonstrated in early works that far...

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Photodynamic therapy (PDT) is a tumor treatment that uses a combination of otherwise non-toxic chemicals, termed photosensitizers (PSs) and visible light to produce reactive oxygen species capable of destroying tumor cells [Dougherty TJ (1993)][Agostinis P. et al.(2011)].PS molecules are often insoluble in water and form aggregates, which...

The possibility of integrating different light-matter interactions to form images in optical microscopy is the starting point for the design and realization of LIQUITOPY®, a ''liquid tunable microscope''. This could represent a new paradigm in data collection and image formation with a potential high impact in biophysics. Liquid takes...

Defects in the global organization of chromatin are relevant in many pathological processes, including cancer. The activation of oncogenes has been recognized as a triggering event of alterations in the dynamics of replication, transcription and DNA damage response (DDR), that may lead to increased DNA damage and the onset of genomic...

The possibility of integrating different light-matter interaction ways for producing images in optical microscopy is the starting point for the design and realization of a brand new optical microscope that has been named "liquid tunable microscope". This represents a new paradigm in data collection and image formation with a potential high...

The fact that the size of many molecular complexes is in the range of 10 to 100nm has generated a growing interest for the development of super-resolved fluorescence microscopy techniques. Optical super-resolution is generally achieved by switching on-off the fluorophores by stochastic or targeted approaches [1]. For instance, in stimulated...