DE JONG , Hidde

Qualitative modeling approaches are promising and still underexploited tools for the analysis and design of synthetic circuits. They can make predictions of circuit behavior in the absence of precise, quantitative information. Moreover, they provide direct insight into the relation between the feedback structure and the dynamical properties of...

Although the effect of temperature on microbial growth has been widely studied, the role of proteome allocation in bringing about temperature-induced changes remains elusive. To tackle this problem, we propose a coarse-grained model of microbial growth, including the processes of temperature-sensitive protein unfolding and chaperone-assisted...

Microorganisms have evolved complex strategies for controlling the distribution of available resources over cellular functions. Biotechnology aims at interfering with these strategies, so as to optimize the production of metabo-lites and other compounds of interest, by (re)engineering the underlying regulatory networks of the cell. The...

Synthetic microbial consortia have been increasingly utilized in biotechnology and experimental evidence shows that suitably engineered consortia can outperform individual species in the synthesis of valuable products. Despite significant achievements, though, a quantitative understanding of the conditions that make this possible, and of the...

The bacterium E. coli is widely used to produce recombinant proteins such as growth hormone and insulin. One inconvenience with E. coli cultures is the secretion of acetate through overflow metabolism. Acetate inhibits cell growth and represents a carbon diversion, which results in several negative effects on protein production. One way to...

Microbial physiology exhibits growth laws that relate the macromolecular composition of the cell to the growth rate. Recent work has shown that these empirical regularities can be derived from coarse-grained models of resource allocation. While these studies focus on steady-state growth, such conditions are rarely found in natural habitats,...

Different strains of a microorganism growing in the same environment display a wide variety of growth rates and growth yields. We developed a coarse-grained model to test the hypothesis that different resource allocation strategies, corresponding to different compositions of the proteome, can account for the observed rate-yield variability. The...

The growth of microorganisms is controlled by strategies for the dynamical allocation of available resources over different cellular functions. Synthetic biology approaches are considered nowadays to artificially modify these strategies and turn microbial populations into biotechnological factories for the production of metabolites of interest....

Microbial growth consists of the conversion of nutrients from the environment into biomassand small energy cofactors (ATP, NADH, NADPH, ...) driving biomass synthesis forward. Twomacroscopic criteria for characterizing microbial growth are growth rate and growth yield. Theformer refers to the rate of conversion of substrate into biomass, and...

Abstract Different strains of a microorganism growing in the same environment display a wide variety of growth rates and growth yields. We developed a coarse-grained model to test the hypothesis that different resource allocation strategies, corresponding to different compositions of the proteome, can account for the observed rate-yield...

Different strains of a microorganism growing in the same environment display a wide variety of growth rates and growth yields. We developed a coarse-grained model to test the hypothesis that different resource allocation strategies, corresponding to different compositions of the proteome, can account for the observed rate-yield variability. The...

Gaining a better comprehension of the growth of microorganisms is a major scientific challenge, which has often been approached from a resource allocation perspective. Simple mathematical self-replicator models based on resource allocation principles have been surprisingly effective in accounting for experimental observations of the growth of...

Gaining a better comprehension of the growth of microorganisms is a major scientific challenge, which has often been approached from a resource allocation perspective. Simple mathematical self-replicator models based on resource allocation principles have been surprisingly effective in accounting for experimental observations of the growth of...

The growth of microorganisms involves the conversion of nutrients in the environment into biomass, mostly proteins and other macromolecules. This conversion is accomplished by networks of biochemical reactions cutting across cellular functions, such as metabolism, gene expression, transport and signalling. Mathematical modelling is a powerful...

Recommandations de la Commission d'Évaluation Inria, adoptées le 25 janvier 2023, sur certains éditeurs, notamment MDPI et Frontiers, qui prennent depuis quelques années une place significative dans les domaines de l'informatique et des mathématiques, disciplines où ils étaient absents par le passé.